ANÁLISIS PRÁCTICO DE LOS REQUISITOS DE CONSTITUCIÓN DE UNA EMPRESA BAJO LA FORMA DE SOCIEDAD DE RESPONSABILIDAD LIMITADA

El presente trabajo trata sobre los diferentes trámites a realizar en el procedimiento de constitución de una sociedad de responsabilidad limitada

Neuro-fuzzy based maneuver detection for collision avoidance in road vehicles

The issue of collision avoidance in road vehicles has been investigated from many different points of view. An interesting approach for Road Vehicle Collision Assistance Support Systems (RVCASS) is based on the creation of a scene of the vehicles involved in a potentially conflictive traffic situation. This paper proposes a neuro-fuzzy approach for dynamic classification of the vehicles roles in a scene. For that purpose, different maneuver state models for longitudinal movements of road vehicles have been defined, and a prototype has been equipped with INS (Inertial Navigation Systems) and GPS (Global Positioning System) sensors. Trials with real data show the suitability of the proposed neurofuzzy approach for solving support to the problem under consideration.Ministerio de Fomento de España y la Agencia Espacial Europea (ESA) patrocinadores de la actividad FOM/3929/2005 and GIROADS 332599 respectivamente

Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.-- et al.[Background]: Fertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes. The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization. [Methods]: For the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16–20 hours; c) only capacitated sperm after 16–20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated. [Results]: The metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC. [Conclusions]: LPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction.This study was supported by grant GV/2009/097 from Department of Education, Generalitat Valenciana, Vicerrectorado de Investigación, University of Alicante, Alicante, Spain (Vigrob-137), the Spanish Ministerio de Economía y Competitividad AGL2012-40180-C03-02 and Fundación Seneca (04542/GERM/06).Peer Reviewe

DNA catabolites in triathletes: effects of supplementation with an aronia–citrus juice (polyphenols-rich juice)

In this study we analyzed whether our aronia–citrus juice (ACJ, the composition is based on a mixture of 95% citrus juice with 5% of Aronia melanocarpa juice), rich in polyphenols, and physical exercise had an effect on seven catabolites of DNA identified in plasma and on a urine isoprostane (8-iso-PGF2α). Sixteen elite triathletes on a controlled diet for triathlon training (45 days) were used in this clinical trial. Our results show a decrease in the 8-hydroxy-2′-deoxyguanosine concentration due to chronic physical exercise. The ACJ intake and physical exercise maintained the guanosine-3′,5′-cyclic monophosphate plasmatic concentrations and decreased the concentration of 8-hydroxyguanine as well as urinary values of 8-iso-PGF2α. Finally, we observed a significant increase in the 8-nitroguanosine levels in triathletes after ACJ intake, compared to the placebo stage. It is concluded that the combination of the intake of ACJ, rich in polyphenolic compounds, with adequate training was able to influence the plasmatic and urinary values of oxidative stress biomarkers. This suggests a positive effect on the oxidative damage and potential associations with DNA repair mechanisms.LAGF was awarded a pre-doctoral FPI fellowship (BES2012-060185) by the Spanish government. This study was supported by the project AGL2011-23690 (CICYT) (Spanish Ministry of Economy and Competitiveness). This work has been partially funded by the “Fundación Séneca de la Región de Murcia” Grupo de Excelencia 19900/GERM/15

Hidratación e ingredientes químicos en el deporte: seguridad alimentaria en el contexto europeo

Before, during and after physical activity, hydration is a limiting factor in athletic performance. Therefore, adequate hydration provides benefits for health and performance of athletes. Besides, hydration is associated to the intake of carbohydrates, protein, sodium, caffeine and other substances by different dietary aids, during the training and/or competition by athletes. These requirements have led to the development of different products by the food industry, to cover the nutritional needs of athletes. Currently in the European context, the legal framework for the development of products, substances and health claims concerning to sport products is incomplete and scarce. Under these conditions, there are many products with different ingredients out of European Food Safety Authority (EFSA) control where claims are wrong due to no robust scientific evidence and it can be dangerous for the health. Further scientific evidence should be constructed by new clinical trials in order to assist to the Experts Commitees at EFSA for obtaining robust scientific opinions concerning to the functional foods and the individual ingredients for sport population.Antes, durante y después de la actividad física, la hidratación es un factor limitante en el rendimiento deportivo. Por lo tanto, una adecuada hidratación proporciona beneficios para la salud y el rendimiento de los deportista. Además, la hidratación se asocia a la ingesta de hidratos de carbono, proteínas, sodio, cafeína y otras sustancias durante el entrenamiento y/o competición de los deportistas. Estos requisitos han llevado al desarrollo de diferentes productos por parte de la industria alimentaria, para cubrir las necesidades nutricionales de los deportistas. Actualmente en el contexto europeo, el marco legal para el desarrollo de productos, sustancias y declaraciones nutricionales y de propiedades saludables relativas a productos deportivos, es incompleta y escasa. Hay muchos productos con diferentes ingredientes controlados por la Agencia Europea de Seguridad Alimentaria (EFSA), los cuales poseen declaraciones nutricionales y de propiedades saludables erróneas, debido a la falta o escasa evidencia científica, resultado peligroso para la salud. Se necesita mayor evidencia científica obtenida a través de nuevos ensayos clínicos con el fin de ayudar a los Comités de expertos de la EFSA para la obtención de dictámenes científicos sólidos relativos a los alimentos funcionales y los ingredientes individuales para la población deportiva.AGI and SM are grateful to the National funding agencies, through the Projects AGL2011-23690 (CICYT), CSD007-0063 (CONSOLIDER-INGENIO 2010 ‘Fun-C-Food’), and Consejo Superior de Investigaciones Científicas (CSIC) 201170E041, to the Spanish Ministry of Economy and Competitiveness, and for the support of the Fundación Séneca – Comunidad Autónoma de la Región de Murcia ‘Group of Excellence in Research’ 04486/GERM/06. Sonia Medina Escudero is appointed under a CSIC research contract

Analysis of health claims regarding creatine monohydrate present in commercial communications for a sample of European sports foods supplements

Objective: To analyse the information on health claims present in the labelling of creatine monohydrate (CM) products. Design: A descriptive study of a selection of products marketed as CM, with health claims, and that met the inclusion/exclusion criteria, was conducted using the Amazon and Google Shopping websites. The adequacy and compliance of the health claims were evaluated with the European legislative requirements (European Food Safety Authority and European Commission). The results were discussed with scientific evidence criteria from the Academy of Nutrition and Dietetics, International Olympic Committee, and International Society of Sports Nutrition, as well as the systematic review carried out by Mielgo-Ayuso in 2019. Setting: Health claims in the commercial communications of a sample of CM supplements, in relation to current legislation and scientific knowledge. Participant: A total of 554 CM products were obtained. Results: Of the total sample, only 167 met the inclusion/exclusion criteria. Of these, 30·5 % recommended a CM dose of 5·0–5·9 g/d, while 29·9 % recommended 3·0 to 3·9 g/d. As for the health claims, ‘Enhances physical performance’ appeared in 73·1 % of the supplements, in most cases referring to a dosage of 3·0 to 3·9 g/d for these products. The rest of the declarations were not adequate or needed to be modified. Conclusion: Only 25 % of the health claims complied with the criteria established by the scientific reference documents. Most of the declarations must be modified or eliminated, as they could be considered fraudulent and/or misleading for the consumer

Consumo de suplementos deportivos en corredores de montaña: diferencias en función del sexo y del nivel competitivo

Introduction: sports supplements (SS) are widely used by all types of athletes to improve their performance. These SS are classified according to the ABCD system of the Australian Institute of Sports (AIS) from higher to lower scientific evidence. In mountain runners, their use could be necessary due to the physiological demands required by this sport. However, the literature on the use of SS by mountain runners is scarce. Objective: to analyze the pattern of SS consumption in mountain runners by studying differences according to sex and competitive level (regional vs national). Methodology: this was a descriptive and cross-sectional study on the consumption and use of SS by mountain runners participating in the Alcoy Solidarity Trail. Data were collected through a validated questionnaire based on content, applicability, structure, and presentation. This questionnaire was completed online by the athletes, who could fill it out voluntarily and at their convenience, as well as anonymously. Results: the results showed that 87.5 % of participants reported consuming SS, with no significant differences observed with respect to competitive level, although differences were found with respect to sex (92.7 % in men vs 70.6 % in women; p = 0.029), with a higher consumption found in men compared to women. The most consumed SS were sports bars (81.9 %), sports drinks (75.0 %), caffeine (48.6 %), magnesium (38.9 %), and electrolytes (27.8 %). Conclusions: among mountain runners consumption of SS is high, and 4 of the 5 most habitually consumed SS belong in the category of greater scientific evidence.Introducción: los suplementos deportivos (SS) son muy utilizados por todo tipo de deportistas para mejorar su rendimiento. Estos SS se clasifican según el sistema ABCD del Australian Institute of Sports (AIS) de mayor a menor evidencia científica. En corredores de montaña, su uso podría ser necesario debido a las exigencias fisiológicas de este deporte. Sin embargo, la literatura sobre el uso de SS por corredores de montaña es escasa. Objetivo: analizar el patrón de consumo de SS en corredores de montaña, estudiando las diferencias según el sexo y el nivel competitivo (autonómico vs. nacional). Metodología: se trata de un estudio descriptivo y transversal sobre el consumo y uso de SS por parte de los corredores de montaña participantes en el Trail Solidario de Alcoy. Los datos fueron recolectados a través de un cuestionario validado en base al contenido, la aplicabilidad, la estructura y la presentación. El cuestionario fue completado de manera online por los atletas que pudieron rellenarlo voluntariamente y a conveniencia, y de manera anónima. Resultados: los resultados mostraron que el 87,5 % de los participantes reportaron consumir SS, no observándose diferencias significativas con respecto al nivel competitivo, aunque sí se encontraron diferencias con respecto al sexo (92,7 % en hombres y 70,6 % en mujeres; p = 0,029), donde los hombres tenían un mayor consumo en comparación con las mujeres. Los SS más consumidos fueron las barritas deportivas (81,9 %), las bebidas deportivas (75,0 %), la cafeína (48,6 %), el magnesio (38,9 %) y los electrólitos (27,8 %). Conclusiones: entre los corredores de montaña, el consumo de SS es alto y 4 de los 5 SS más consumidos pertenecen a la categoría de mayor evidencia científica

Phytoprostanes determination in almonds depending on the cultivar and growing conditions (conventional versus ecological)

[SPA] En el año 2000, se descubrieron unos compuestos denominados fitoprostanos obtenidos por la oxidación no enzimática del ácido α- linolénico. Las almendras contienen un alto contenido en ácidos grasos poliinsaturados siendo el ácido α- linolénico uno de los más importantes para la salud. En este trabajo se propone la cuantificación de fitoprostanos en almendras y averiguar si éstos están influidos por el tipo de variedad y condiciones de cultivo (convencional frente a ecológico). Como resultado, mencionar que se encontraron 7 tipos de fitoprostanos aunque no todas las variedades los presentaban. El fitoprostano 9–F1t-Phytop fue el más abundante, aunque también se obtuvieron otros fitoprostanos en menor concentración tipo 9-epi-9E1t-PhytoP, 9-E1t-PhytoP, 16-B1-phytoP y 9-L1-PhytoP. Se obtuvo una influencia en función del tipo cultivo siendo “Marcona” y “Largueta” las variedades que presentaron mayor contenido de fitoprostanos en cultivo convencional. Sin embargo, las variedades, Planeta”, “Colorada”, “Atocha”, “Garriges” y “Avellanera”, mostraron una mayor concentración en cultivo ecológico. [ENG] In 2000, compounds named Phytoprostanes (phytoP) generated by non-enzymatic oxidation of α-linolenic acid were discovered. Almonds kernels have a high content of polyunsaturated fatty acids being the α-linolenic acid, one of the most important fatty acids for human health. In this work the PhytoP quantification in almonds is proposed and it is studied the cultivar and growing conditions (conventional versus ecological). Results showed the existence of seven types of PhytoP although not all cultivars contained all of them. 9-F1t-PhytoP was the most abundant, although others PhytoPs were also obtained in lower concentrations as type 9-epi-9E1t-PhytoP, 9-E1t-PhytoP, 16-B1-phytoP and 9-L1-PhytoP. A cultivar influence combined with growing conditions was found. ‘Marcona’ and ‘Largueta’ cultivars, under conditional conditions, showed the higher total PhytoPs content. In contrast, ‘Planeta’, ‘Colorada’, ‘Atocha’, ’Garriges’ and ‘Avellanera’ cultivars showed a greater concentration under ecological conditions.Se agradece al Instituto de Biotecnología Vegetal de la UPCT y al CEBAS-CSIC la utilización de algunos equipos. Queremos destacar la colaboración desinteresada de la Cooperativa COATO (Totana, Murcia) por su aportación varietal

Relationship between the Ingestion of a Polyphenol-Rich Drink, Hepcidin Hormone, and Long-Term Training

The effects of polyphenol-rich foods on the iron status of athletes, as well as the effect of physical training on the hormone hepcidin, implicated in iron metabolism, are not clear. We investigated the influence on iron metabolism of a long-term training intervention of 120 days, measuring the hepcidin concentration in the plasma of 16 elite triathletes, and the effect of the ingestion of 200 mL of either aronia-citrus juice or a placebo drink for 45 days, in a crossover design. The highest plasma hepcidin concentrations were observed at the beginning of the study (116 ± 63 nM) and levels steadily decreased until the end of the intervention (final value 10 ± 7.5 nM). Long-term training might reduce inflammation and, hence, could be responsible for the decrease in hepcidin in triathletes. Polyphenols from aronia-citrus juice did not interfere in iron absorption, as we did not observe significant differences between the intake of the placebo drink or juice with regard to hepcidin levels. Further studies are required to ascertain the time and conditions necessary to restore hepcidin levels, which reflect the iron status of triathletes.The study received financial support from the National Funding Agencies, through the Project AGL2011-23690 (CICYT). Sonia Medina is grateful to the CICYT for a research contract (AGL2011-23690). This work has been partially funded by the “Fundación Séneca de la Región de Murcia” Grupo de Excelencia 19900/GERM/15

¿Todo mejora el rendimiento deportivo? Lagunas legales de los suplementos ergonutricionales

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist